Cell culture:. Culture and preservation of endothelial and different cancer cell linesviz.EA.hy926 ,MCF-7, stem cells, primary cell culturing.
Animal handling: Rats and mice
In vitro studies: cell proliferation, cell cycle study, nuclear apoptosis, DNA fragmentation, ROS generation ,antioxidant activity,MC540 , PI and nitric oxide production, flow cytometry, telomerase assay.
Molecular analysis: Isolation and estimation of genomic DNA/RNA, cDNA synthesis, PCR amplification, Restriction digestion, carried out agarose and polyacrylamide gel electrophoresis and quantitative real-time PCR of expression analysis , LCMS, Immunoprecipitation.
Histological study: Cryosectioning of heart tissue of rat and hypertrophy study.
Microbial techniques: Isolation and identification of antibiotic sensitive bacteria, maintenance of microbiological strains, plasmid isolation from microbial cells, cloning and transformation.
Proteomics: Isolation and estimation of proteins, PAGE-SDS & Native.
Bioinformatics and computer skills: Knowledge of computational and statistical analysis of biological systems by GraphPad Prism 5, BLAST, Primer design using NCBI and other web based bioinformatics tools.
Plant Pathology: Experience in plant pathology, Plant disease, Fungal pathogen identification, Plant disease management, molecular plant-microbe interactions, plant/fungal miRNA, transcriptomic analysis
CRISPR/CAS9 genome editing
Genome sequencing DNA methylation analysis
Dermal toxicity and wound healing activity : Invitro and invivo models
Biocompatibility testing: Cytotoxity, neurotoxicity, irritation and intracutaneous activity
Microscopy: cellular imaging (compound , fluorescent and confocal microscope)
Team work and learning new skills.
Expected Base Salary
Total Professional Experience
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